The Total Inhibin enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of Total inhibin (Inhibin A, Inhibin B and Inhibin alpha subunit) in human serum, plasma and other biological fluids.
| Catalog Number | |
|---|---|
| Inventory Status | Made to order. Inquire about leadtime. |
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 6, 8.3-525 pg/mL |
| Limit of Detection | 0.94 pg/mL |
| Sample Size | 50 µL |
| Sample Type | Plasma, Serum |
| Assay Time | 3.5 hours |
| Shelf Life | 24 months |
| Species Reactivity | Human |
| Availability | Worldwide |
Inhibins are heterodimeric protein hormones secreted by granulosa cells of the ovary in the female and Sertoli cells of the testis in the male. They selectively suppress the secretion of pituitary follicle stimulating hormone (FSH) and also have local paracrine actions in the gonads. The fully processed form of the inhibin molecule has a molecular weight of approximately 32 kD and consists of the two distinct chains (α and β), linked by disulfide bridges. Higher molecular weight forms, with precursor forms of the α-subunit, also occur in follicular fluid and serum. In addition, free α-subunit forms, unassociated with a β-subunit, and lacking inhibin bioactivity, are also present. Measurements of Inhibins are shown to be useful in studying its role in human reproductive physiology, spermatogenesis, testicular function, ovarian reserve, granulosa cell tumors and mucinous tumors.
References:
1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
2. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.
4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
Inhibin (Total) ELISA AL-134
Priyanka S, Laura J, Ben H, Victoria A, Shrikant P, Prabhjot K, Tapasya S, Nadine H, Balázs G, Eugenia B, Nam L, Karthikeyan M. Inhibin is a novel paracrine factor for tumor angiogenesis and metastasis. Cancer Res. 2018 June 01; 78(11): 2978–2989. doi:10.1158/0008-5472.CAN-17-2316
All Products Cited: Inhibin (Total) ELISA AL-134
Kristensen SG, Kumar A, Kalra B, Pors SE, Bøtkjær JA, Mamsen LS, Colmorn LB, Fedder J, Ernst E, Owens LA, Hardy K, Franks S, Andersen CY. Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO. J Clin Endocrinol Metab. 2019 Dec 1;104(12):6371-6384. doi:10.1210/jc.2019-01094. PMID: 31287539.
All Products Cited: GDF-9 ELISA AL-179; picoAMH ELISA AL-124; Inhibin-A ELISA AL-123; Inhibin-B ELISA AL-107; Inhibin (Total) ELISA AL-134; Activin-A ELISA AL-110; Activin-B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; FTSL-3 ELISA AL-152
Erickson JA, Grenache DG. Performance evaluation of a total inhibin ELISA and reference intervals in female and male populations. Poster session presented at American Association of Clinical Chemistry 2017; July 30-Aug 3, San Diego, CA.
All Products Cited: Total Inhibin ELISA AL-134
Lambert-Messerlian G, Steinhoff M, Kumar A, Eklund EE, Moore R. Comparison of Inhibin Alpha Subunit and Antimüllerian Hormone Immunoreactivity in Granulosa Cell and Mucinous Ovarian Tumors. Appl Immunohistochem Mol Morphol. 2017 Jan;25(1):71-77.
All Products Cited: Inhibin alpha antibodies; AMH antibodies; Total Inhibin ELISA AL-134; AMH ELISA AL-105
Calvert ME, Kalra B, Patel A, Kumar A, Shaw ND. Serum and urine profiles of TGF-β superfamily members in reproductive aged women. Clin Chim Acta. 2021 Dec 5;524:96-100. doi: 10.1016/j.cca.2021.12.002. Online ahead of print
All Products Cited: Inhibin A (pico) ELISA AL-184; Inhibin B ELISA AL-107; Inhibin (Total) ELISA AL-134; AMH (PCOCheck) ELISA AL-196; Activin A ELISA AL-110; Activin B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; GDF-9/BMP-15 Complex ELISA AL-181; GDF-15 (Total) ELISA AL-1014; GDF-15 (H2O2D non-detectable) ELISA AL-1018
Kristensen SG, Kumar A, Mamsen LS, Kalra B, Pors SE, Bøtkjær JA, Macklon KT, Fedder J, Ernst E, Hardy K, Franks S, Andersen CY. Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries. J Clin Endocrinol Metab. 2022 May 2:dgac272. doi: 10.1210/clinem/dgac272. Epub ahead of print. PMID: 35511085.
All Products Cited: GDF-9 ELISA AL-176; BMP-15 ELISA AL-179; GDF-9/BMP-15 Complex ELISA AL-181; AMH ELISA AL-105; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Total Inhibin ELISA AL-134; Activin B ELISA AL-110; Activin AB ELISA AL-153; Follistatin ELISA AL-117