The Rat and Mouse Anti-Müllerian hormone (AMH) enzyme-linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of AMH in rat and mouse serum and other biological fluids.
| Catalog Number | |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 2, 0.313-20 ng/mL |
| Limit of Detection | TBD |
| Sample Size | 5 µL Predilution |
| Sample Type | Serum |
| Assay Time | 3.5 hours |
| Shelf Life | 24 months |
| Species Reactivity | Alpaca Serum, Beluga Whale Serum, Bottlenose Dolphin Serum, Hamster Serum, Murine Serum, Rabbit Serum, Rattus Serum, Vervet Monkey Serum, White Tailed Deer Serum |
| Availability | Worldwide |
Anti-Müllerian hormone is a 140 kDa glycoprotein that is produced during normal embryogenesis by the Sertoli cells of the embryonic testis, causes the involution of the Müllerian duct, and inhibits female gonadogenesis by inducing apoptosis of target gonadal cells. It belongs to the transforming growth factor-β super family. AMH causes apoptosis of specific Anti-Müllerian inhibiting substance (MIS) receptor-bearing cells, while having no effect on cells without receptors. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary, and AMH inhibits recruitment of primordial follicles into the pool of growing follicles, and decreases responsiveness of growing follicles to FSH.
References:
1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
2. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.
4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
AMH (Rat/Mouse) ELISA AL-113
Aiken CE, Tarry-Adkins JL, Ozanne SE. Transgenerational Developmental Programming of Ovarian Reserve. Sci Rep. 2015 Nov 3;5:16175. doi: 10.1038/srep16175
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Aiken CE, Tarry-Adkins JL, Ozanne SE. Transgenerational Developmental Programming of Ovarian Reserve. Scientific Reports, 5:16175. Published 2015 Nov 3.
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Arroyo E, Patiño C, Ciccarelli M, Raudsepp T, Conley A, Tibary A. Clinical and Histological Features of Ovarian Hypoplasia/Dysgenesis in Alpacas. Front Vet Sci. 2022 Mar 25;9:837684. doi: 10.3389/fvets.2022.837684. PMID: 35400100; PMCID: PMC8990812.
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Barrett, C. Effects of Photoperiod on Novelty Preference in Preadolescent Siberian Hamster (Phodopus sungorus) Pups. [Thesis, 2014]. Ithaca (NY)]: Cornell University.
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Becker M, Haluska P, Bale L, Oxvig C, Conover C. A Novel Neutralizing Antibody Targeting Pregnancy-Associated Plasma Protein-A Inhibits Ovarian Cancer Growth and Ascites Accumulation in Patient Mouse Tumorgrafts. Mol Cancer Ther. 2015 Apr;14(4):973-81. Epub 2015 Feb 18.
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Bourgneuf C, Bailbé D, Lamazière A, Dupont C, Moldes M, Farabos D, et al. The Goto-Kakizaki rat is a spontaneous prototypical rodent model of polycystic ovary syndrome. Nat Commun. 2021;12(1064):1-13. doi:10.1038/s41467-021-21308-y.
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Carolino ROG, Barros PT, Kalil B, Anselmo-Franci J. . Endocrine profile of the VCD-induced perimenopausal model rat. PLoS One. 2019 Dec 30;14(12):e0226874. doi: 10.1371/journal.pone.0226874
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Devillers MM, Petit F, Cluzet V, François CM, Giton F, Garrel G, Cohen-Tannoudji J, Guigon CJ. FSH inhibits AMH to support ovarian estradiol synthesis in infantile mice. J Endocrinol. 2019 Feb 1;240(2):215-228. doi: 10.1530/JOE-18-0313. PMID: 30403655.
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Dopavogui L, Cadoret F, Loison G, El Fouikar S, Frenois FX, Giton F, et al. Pre- and postnatal dietary exposure to a pesticide cocktail disrupts ovarian functions in 8-week-old female mice. Int J Mol Sci. 2022;23(7525):1-17. doi:10.3390/ijms23147525.
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Habara O, Logan CY, Kanai-Azuma M, Nusse R, Takase HM. WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility. Development. 2021 May 1;148(9):dev198846. doi: 10.1242/dev.198846
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Hoek J, van Houten E, Kramer P, McLuskey A, Karels B, Themmen A, Visser A. Analysis of the Metabolic Phenotype of Female Mice Lacking Anti-Mullerian Hormone Signaling. Poster session presented at the 96th Annual Endocrine Society Meeting; 2014 Jun 21-24; Chicago, IL.
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Hohos NM, Elliott EM, Cho KJ, Lin IS, Rudolph MC, Skaznik-Wikiel ME. . High-Fat Diet-Induced Dysregulation of Ovarian Gene Expression is Restored with Chronic Omega-3 Fatty Acid Supplementation. Mol Cell Endocrinol. 2020 Jan 1;499:110615. doi: 10.1016/j.mce.2019.110615
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Holland S, Prescott M, Pankhurst M, Campbell RE. The infuence of maternal androgen excess on the male reproductive axis. Sci Rep . 2019 Dec 11;9(1):18908. doi: 10.1038/s41598-019-55436-9.
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Kalem MN, Anadol E, Kalem Z, et al. A rat study on the PTEN expression in ovarian tissue in PCOS and folliculogenesis. Sci Rep. 2023 Dec;13:20774. doi: 10.1038/s41598-023-47809-y.
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Kumar A, Kalra B, Patel A, Shah S. New Sensitive Anti-Müllerian Hormone (AMH) ELISAs for Non-Human Primate, Rodent, Equine, Bovine, Canine and Other Species. Poster session presented at: American Association for Clinical Chemistry; 2013; Jul 30-Aug 1; Houston, TX.
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Luba Sominsky, Simin Younesi, Simone N De Luca, Sophie M Loone, Kylie M Quinn, Sarah J Spencer, Ovarian follicles are resistant to monocyte perturbations—implications for ovarian health with immune disruption, Biology of Reproduction, Volume 105, Issue 1, July 2021, Pages 100–112, https://doi.org/10.1093/biolre/ioab049
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Mohamed SA, Shalaby SM, Abdelaziz M, Brakta S, Hill WD, Ismail N, Al-Hendy A. Human Mesenchymal Stem Cells Partially Reverse Infertility in Chemotherapy-Induced Ovarian Failure. Reprod Sci. 2017 Jan 1:1933719117699705.
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Montano GA, Robeck TR, Steinman KJ, O’Brien JK. Circulating anti-Müllerian hormone concentrations in relation to age and season in male and female beluga (Delphinapterus leucas). Reprod Fertil Dev.
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Razawy W, Alves CH, Koedam M, Asmawidjaja PS, Mus AMC, Oukka M, Leenen PJM, Visser JA, van der Eerden BCJ, Lubberts E. IL‑23 receptor defciency results in lower bone mass via indirect regulation of bone formation. Sci Rep. 2021 May 13;11(1):10244. doi: 10.1038/s41598-021-89625-2. PMID: 33986359; PMCID: PMC8119722.
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Robert T Rydze, Bethany K Patton, Shawn M Briley, Hannia Salazar Torralba, Gregory Gipson, Rebecca James, Aleksandar Rajkovic, Thomas Thompson, Stephanie A Pangas, Deletion of Gremlin-2 alters estrous cyclicity and disrupts female fertility in mice, Biology of Reproduction, Volume 105, Issue 5, November 2021, Pages 1205–1220, https://doi.org/10.1093/biolre/ioab148
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Rodriguez A, Briley SM, Patton BK, Tripurani SK, Rajapakshe K, Coarfa C, Rajkovic A, Andrieux A, Dejean A, Pangas SA. . Loss of the E2 SUMO-conjugating enzyme Ube2i in oocytes during ovarian folliculogenesis causes infertility in mice
. Development. 2019 Dec 2;146(23):dev176701. doi: 10.1242/dev.176701
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Rodriguez KF, Brown PR, Amato CM, Nicol B, Liu CF, Xu X, Yao HH. Somatic cell fate maintenance in mouse fetal testes via autocrine/paracrine action of AMH and activin B. Nat Commun. 2022;13(4130):1-12. doi:10.1038/s41467-022-31486-y.
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Roy S, Gandra D, Seger C, Biswas A, Kushnir VA, Gleicher N, Kumar TR, Sen A. Oocyte-Derived Factors (GDF9 and BMP15) and FSH Regulate AMH Expression Via Modulation of H3K27AC in Granulosa Cells. Endocrinology. 2018 Sep 1;159(9):3433-3445.
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Ruohonen ST, Gaytan F, Usseglio Gaudi A, Velasco I, Kukoricza K, Perdices-Lopez C, Franssen D, Guler I, Mehmood A, Elo LL, Ohlsson C, Poutanen M, Tena-Sempere M. Selective loss of kisspeptin signaling in oocytes causes progressive premature ovulatory failure. Hum Reprod. 2022 Apr 1;37(4):806-821. doi: 10.1093/humrep/deab287. PMID: 35037941; PMCID: PMC8971646.
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Ruth KS, Day FR, Hussain J, et al. Genetic insights into biological mechanisms governing human ovarian ageing. Nature. 2021 Aug;596(7872):393-397. doi: 10.1038/s41586-021-03779-7. Epub 2021 Aug 4
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Sara M, Shahinaz S, Soumia B, Lelyand E, Amro E, Ayman A. Umbilical Cord Blood Mesenchymal Stem Cells as an Infertility Treatment for Chemotherapy Induced Premature Ovarian Insufficiency. Biomedicines. 2019 Jan 18;7(1):7. doi: 10.3390/biomedicines7010007
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Silva MSB, Decoster L, Delpouve G, Lhomme T, Ternier G, Prevot V, Giacobini P. Overactivation of GnRH neurons is sufficient to trigger polycystic ovary syndrome-like traits in female mice. eBioMedicine. 2023;97:104850. doi:10.1016/j.ebiom.2023.104850.
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Wang Z, Shen M, Xue P, DiVall SA, Segars J, Wu S. Female Offspring From Chronic Hyperandrogenemic Dams Exhibit
Delayed Puberty and Impaired Ovarian Reserve. Endocrinology. 2018 Feb 1;159(2):1242-1252. doi: 10.1210/en.2017-03078
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Winship AL, Griffiths M, Lliberos Requesens C, Sarma U, Phillips KA, Hutt KJ. The PARP inhibitor, olaparib, depletes the ovarian reserve in mice: implications for fertility preservation. Hum Reprod. 2020 Aug 1;35(8):1864-1874. doi: 10.1093/humrep/deaa128. PMID: 32604417.
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Ygnacio RHC. Determinación de la Reserva Ovárica en Alpacas Mediante la Concentración de la Hormona Anti-Mülleriana (AMH) en el Plasma Sanguíneo. [Thesis, 2017]. Lima (Peru)]: Universidad Nacional Agraria la Molina Escuela de Posgrado Maestría en Producción Animal.
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Zheng M, Cadenas J, Pors SE, Esa T, Kristensen SG, Mamsen LS, Adrados CS, Andersen CY. Reducing 3D hydrogel stiffness, addition of oestradiol in a physiological concentration, and increasing FSH concentration improve in vitro growth of murine preantral follicles. Int J Mol Sci. 2023;24(12499):1-13. doi:10.3390/ijms241512499.
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Zhou Y, Scott KL, Quin E, Pankhurst MW. Serum Anti-Müllerian Hormone Is an Effective Indicator of Antral Follicle Counts but Not Primordial Follicle Counts. Endocrinology. 2023 Jun 26;164(8):bqad098. doi: 10.1210/endocr/bqad098. PMID: 37337678; PMCID: PMC10318615.
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