The Ultra-Sensitive Inhibin B enzyme linked immunuosorbent assay (ELISA) kit provides materials for the quantitative measurement of Inhibin B in human serum and other biological fluids.
| Catalog Number | AL-195 (RUO) Instructions for Use |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 6, 3.9-542 pg/mL |
| Limit of Detection | 0.77 pg/mL |
| Sample Size | 50 µL |
| Sample Type | Serum |
| Assay Time | 1.5 hours |
| Shelf Life | 24 months |
| Species Reactivity | Human |
| Availability | Worldwide |
| Analyte | Inhibin B |
Inhibin B is a dimeric hormone that is composed of alpha (α) and beta B (βB) subunits. The free alpha subunits usually do not have any physiological effect. Therefore, the bioactivity of the inhibins depends on the formation of a dimeric α-β structure, and only dimeric forms of inhibins are biologically active. Inhibins are protein hormones secreted by granulosa cells of the ovary in the female and sertoli cells of the testis in the male. They selectively suppress the secretion of pituitary follicle stimulating hormone (FSH) and also have local paracrine actions in the gonads. Inhibin B levels have been reported in sertoli cell function (potential marker for spermatogenesis and testicular function), ovarian reserve and granulosa cell tumors.
References:
1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
2. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.
4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
Inhibin B (Ultra-Sensitive) ELISA AL-195
Barbotin AL, Kumar A, Kalra B, Savjani G, Mitchell V, Pigny P. SAT-043 Utility of Ultrasensitive Inhibin B Measurement for the Management of Men with Non-Obstructive Azoospermia. Journal of the Endocrine Society, Volume 4, Issue Supplement_1, April-May 2020, SAT-043, https://doi.org/10.1210/jendso/bvaa046.659
All Products Cited: Inhibin B Ultra Sensitive ELISA AL-195
Barbotin AL, Kumar A, Kalra B, Savjani G, Mitchell V, Pigny P. Utility of ultrasensitive inhibin B measurement for the management of men with non-obstructive azoospermia. J Endocr Soc. 2020;4(Supplement_1). doi:10.1210/jendso/bvaa046.
All Products Cited: Inhibin B (Ultra-Sensitive) ELISA AL-195
Danis RB, Sriprasert I, Ho J, Kumar A, McGinnis L, Stanczyk F. Bioavailable Inhibin B (Inh B)may be a better marker of oocyte yield than currently used markers of ovarian reserve. Fertility and Sterility Volume 114, Issue 3, Supplement, E453, September 01, 2020 DOI: https://doi.org/10.1016/j.fertnstert.2020.08.1308
All Products Cited: Inhibin B (Ultra-Sensitive) ELISA AL-195
Luu TH, Kuhn K, Bradford AP, Johnson R, Carlson N, Kumar TR, Polotsky AJ. Obese women exhibit reduced inhibin B and estradiol secretion following pulsatile intravenous FSH administration. J Endocr Soc. 2021;5(Supplement_1). doi:10.1210/jendso/bvab048.
All Products Cited: Inhibin B (Ultra-Sensitive) ELISA AL-195