The Inhibin B enzyme linked immunuosorbent assay (ELISA) kit provides materials for the quantitative measurement of Inhibin B in human serum and other biological fluids.
| Catalog Number | AL-107 (RUO) Instructions for Use |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Species Reactivity | Enquire about animal-specific cross-reactivity |
| Dynamic Range | 6, 12.7-1390 pg/mL |
| Limit of Detection | 1.6 pg/mL |
| Sample Size | 50 µL |
| Sample Type | Serum |
| Assay Time | 3.5 hours |
| Shelf Life | 24 months |
| Availability | United States |
Inhibin B is a dimeric hormone that is composed of alpha (α) and beta B (βB) subunits. The free alpha subunits usually do not have any physiological effect. Therefore, the bioactivity of the inhibins depends on the formation of a dimeric α-β structure, and only dimeric forms of inhibins are biologically active. Inhibins are protein hormones secreted by granulosa cells of the ovary in the female and sertoli cells of the testis in the male. They selectively suppress the secretion of pituitary follicle stimulating hormone (FSH) and also have local paracrine actions in the gonads. Inhibin B levels have been reported in sertoli cell function (potential marker for spermatogenesis and testicular function), ovarian reserve and granulosa cell tumors.
References:
1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
2. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.
4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
Inhibin B CLIA AL-207
Attaelmannan M, Pandian R, Thomassian K, Khachatryan S, Kumar A. Development and Validation of an Improved Chemiluminescent Assay for Inhibin B. Poster session presented at: American Association for Clinical Chemistry; 2013; Jul 30-Aug 1; Houston, TX.
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Inhibin B ELISA AL-107
Danis RB, Sriprasert I, Ho JR, M LK, Kumar A, Stanczyk FZ. Association of bioavailable inhibin B and oocyte yield in controlled ovarian stimulation. F&S Reports, Available online 31 March 2021. https://doi.org/10.1016/j.xfre.2021.03.005
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Chinya A, Ratan SK, Aggarwal SK, Garg A, Mishra TK. Association of Levels of Serum Inhibin B and Follicle-stimulating Hormone with Testicular Vascularity, Volume, and Echotexture in Children with Undescended Testes. J Indian Assoc Pediatr Surg. 2017 Jan-Mar;22(1):3-8. doi: 10.4103/0971-9261.194609. PMID: 28082768; PMCID:
PMC5217136
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Lahlou N, Santullli P, Guibourdenche J, Beldjord C, Kottler, M, Chapron C, Gompel A. Unexpected Inhibin B, Insulin-like Peptide 3, and Testosterone Production by Mother Ovaries in a Pregnancy with Sustained High hCG Levels. A Clue to Hyperreactio Luteinalis? . Poster session presented at: Endocrine Society’s 96th Annual Meeting; 2014; June 21-24; Chicago, IL.
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Sharma SK, Jain S, Bahl P, Potturi P, Rathi M, Naidu S, Sachdeva N, Dhir V, Jain S. Ovarian dysfunction with moderate-dose intravenous cyclophosphamide (modified NIH regimen) and mycophenolate mofetil in young adults with severe lupus: a prospective cohort study. Sharma et al. Arthritis Research & Therapy (2020) 22:189 https://doi.org/10.1186/s13075-020-02292-y
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Qiaofei H, Liming H, Mingyue N, Qin W, Ying F, Yinmei D, Yanhong Z, Shuyu W, Chenghong Y, Xiaokui Y. The effect of dehydroepiandrosterone supplementation on ovarian response is associated with androgen receptor in diminished ovarian reserve women. Hu et al. Journal of Ovarian Research (2017) 10:32 DOI 10.1186/s13048-017-
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Du YY, Guo N, Wang YX, Hua X, Deng TR, Teng XM, Yao YC, Li YF. Urinary phthalate metabolites in relation to serum anti-Müllerian hormone and inhibin B levels among women from a fertility center: a retrospective analysis. Du et al. Reproductive Health (2018) 15:33 https://doi.org/10.1186/s12978-018-0469-8
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Calvert ME, Kalra B, Patel A, Kumar A, Shaw ND. Serum and urine profiles of TGF-β superfamily members in reproductive aged women. Clin Chim Acta. 2021 Dec 5;524:96-100. doi: 10.1016/j.cca.2021.12.002. Online ahead of print
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Tencer J, Lemaire P, Brailly-Tabard S, Brauner R. Serum inhibin B concentration as a predictor of age at first menstruation in girls with idiopathic central precocious puberty. PLoS One. 2018 Dec 14;13(12):e0205810. doi:10.1371/journal.pone.0205810. eCollection 2018.
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Guaragna-Filho G, Calixto AR, De Paula GB, De Oliveira LC, Morcillo AM, De Mello MP, Maciel-Guerra AT, Guerra-Junior G. Comparison between two inhibin B ELISA assays in 46,XY testicular disorders of sex development (DSD) with normal testosterone secretion. J Pediatr Endocrinol Metab. 2018 Jan 26;31(2):191-194. doi:
10.1515/jpem-2017-0351. PMID: 29306929.
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Mamsen LS, Charkiewicz K, Anderson RA, Telfer EE, McLaughlin M, Kelsey TW, Kristensen SG, Gook DA, Ernst E, Andersen CY. Characterization of follicles in girls and young women with Turner syndrome who underwent ovarian tissue cryopreservation. Fertil Steril. 2019 Jun;111(6):1217-1225.e3. doi: 10.1016/j.fertnstert.2019.02.003. Epub 2019 Mar 25. PMID: 30922638.
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Manente L, Pecoraro S, Picillo E, Gargiulo U, Gargiulo P, De Luca A, Politano L. Molecular evidence of apoptotic pathway activation in semen samples with high DNA fragmentation. In Vivo. 2015 Mar-Apr;29(2):289-94.
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Liu DM, Torchen LC, Sung Y, Paparodis R, Legro RS, Grebe SK, Singh RJ, Taylor RL, Dunaif A. Evidence for gonadotrophin secretory and steroidogenic abnormalities in brothers of women with polycystic ovary syndrome. Human Reproduction, Vol.29, No.12 pp. 2764–2772, 2014 Oct 21.
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Kim SY, Ebbert K, Cordeiro MH, Romero MM, Whelan KA, Suarez AA, Woodruff TK, Kurita T. Constitutive Activation of PI3K in Oocyte Induces Ovarian Granulosa Cell Tumors. Cancer Res. 2016 Jul 1;76(13):3851-61
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Harris BS, Steiner AZ, Jukic AM. Ovarian Reserve Biomarkers and Menstrual Cycle Length in a Prospective Cohort Study. J Clin Endocrinol Metab. 2021 Aug 18;106(9):e3748-e3759. doi: 10.1210/clinem/dgab204
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Kumar A, Kalra B, Patel A, Shah S. Development of a Sensitive Inhibin B ELISA Optimized to Eliminate False Positive Results. Poster session presented at: American Association for Clinical Chemistry; 2013; Jul 30-Aug 1; Houston, TX.
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Kim A, Ebbert K, Cordeiro MH, Romero M, Zhu J, Serna VA, Whelan KA, Woodruff TK, Kurita T. Cell Autonomous Phosphoinositide 3-Kinase Activation in Oocytes Disrupts Normal Ovarian Function Through Promoting Survival and Overgrowth of Ovarian Follicles. Endocrinology. 2015 Apr; 156(4): 1464–1476.
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Torchen L, Kumar A, Kalra B, Savjani G, Sisk R, Legro R, Dunaif A. Brothers and Fathers of Women with PCOS Have Altered Testicular Function. Poster session presented at: Endocrine Society’s 96th Annual Meeting; 2014; June 21-24; Chicago, IL.
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Istanbullu S, Lahlou N, Chevenne D, Léger J, Carel J, Martinerie L. Constitutional delay of puberty or hypogonadotropic hypogonadism: Diagnostic value of inhibin B and AMH measurements. Abstract presented at 55th European Society for Paediactric Endocrinology; 2016; Sep 10-12; Paris, France.
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Zhou W, Fang F, Zhu W, Chen ZJ, Du Y, Zhang J. Bisphenol A and Ovarian Reserve among Infertile Women with Polycystic Ovarian Syndrome. Int J Environ Res Public Health. 2016 Dec 27;14(1). pii: E18.
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Jain M, Kalsi AK, Srivastava A, Gupta YK, Halder A. High Serum Estradiol and Heavy Metals Responsible for Human Spermiation Defect-A Pilot Study. J Clin Diagn Res. 2016 Dec;10(12):RC09-RC13.
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Xiao S, Coppeta JR, Rogers HB, Isenberg BC, Zhu J, Olalekan SA, McKinnon KE, Dokic D, Rashedi AS, Haisenleder DJ, Malpani SS, Arnold-Murray CA, Chen K, Jiang M, Bai L, Nguyen CT, Zhang J, Laronda MM, Hope TJ, Maniar KP, Pavone ME, Avram MJ, Sefton EC, Getsios S, Burdette JE, Kim JJ, Borenstein JT, Woodruff TK. A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle. Nat Commun. 2017 Mar 28;8:14584.
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Torchen LC, Kumar A, Kalra B, Savjani G, Sisk R, Legro RS, Dunaif A. Increased antim€ullerian hormone levels and other reproductive endocrine changes in adult male relatives of women with polycystic ovary syndrome. Fertil Steril. 2016 Jul;106(1):50-55.
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Kristensen SG, Kumar A, Mamsen LS, Kalra B, Pors SE, Bøtkjær JA, Macklon KT, Fedder J, Ernst E, Hardy K, Franks S, Andersen CY. Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries. J Clin Endocrinol Metab. 2022 May 2:dgac272. doi: 10.1210/clinem/dgac272. Epub ahead of print. PMID: 35511085.
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Finkelstein JS, Lee H, Karlamangla A, Neer RM, Sluss PM, Burnett-Bowie SA, Darakananda K, Donahoe PK, Harlow SD, Prizand SH, Joffe H, Kumar A, Martin DE, McConnell D, Merrilat S, Morrison A, Pastore LM, Randolph JF, Greendale GA, Santoro N. Antimullerian Hormone and Impending Menopause in Late Reproductive Age: The Study of Women’s Health Across the Nation. The Journal of Clinical Endocrinology & Metabolism, Volume 105, Issue 4, April 2020, Pages e1862–e1871, https://doi.org/10.1210/clinem/dgz283
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Legro R, Dodson W, Estes S, Kunselman A, Coutifaris C, Dokras A. Effect of Weight Loss and Hormonal Suppression Alone and in Combination on Bioactive Gut and Ovarian Peptide Hormones in Women with Polycystic Ovary Syndrome. Journal of the Endocrine Society, Volume 3, Issue Supplement_1, April-May 2019, MON-233, https://doi.org/10.1210/js.2019-MON-233
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Kristensen SG, Kumar A, Kalra B, Pors SE, Bøtkjær JA, Mamsen LS, Colmorn LB, Fedder J, Ernst E, Owens LA, Hardy K, Franks S, Andersen CY. Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO. J Clin Endocrinol Metab. 2019 Dec 1;104(12):6371-6384. doi:10.1210/jc.2019-01094. PMID: 31287539.
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Kalra B, Patel A, Kommareddy V, Ganapathy K, Mistry S, Budhavarapu V, Savjani G, Pasternack A, Ritvos O, Hyvonen M, Kumar A. Development of Novel Specific ELISAs for all Inhibins, Activins and their Binding Proteins FST and FSTL3. Poster presented at 98th Annual Endocrine Society Meeting; 2016 Apr 1-3; Boston, MA.
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Corvest V, Lemaire P, Brailly-Tabard S, Brauner R. Puberty and Inhibin B in 35 Adolescents With Pituitary Stalk Interruption Syndrome. Front Pediatr. 2020 Jun 11;8:304. doi: 10.3389/fped.2020.00304. eCollection 2020
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Chaudhary S, Walia R, Bhansali A, Dayal D, Sachdeva N, Singh T, Bhadada SK. . FSH-stimulated Inhibin B (FSH-iB): A Novel Marker for the Accurate Prediction of Pubertal Outcome in Delayed Puberty. J Clin Endocrinol Metab. 2021 Aug 18;106(9):e3495-e3505. doi: 10.1210/clinem/dgab357
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Kano M, Hsu JY, Saatcioglu HD, Nagykery N, Zhang L, Morris Sabatini ME, Donahoe PK, Pépin D. Neoadjuvant Treatment With Mullerian_Inhibiting Substance Synchronizes Follicles and Enhances Superovulation Yield. J Endocr Soc. 2019 Jul 22;3(11):2123-2134. doi: 10.1210/js.2019-00190. eCollection 2019 Nov 1
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Perakakis N, Upadhyay J, Ghaly W, Chen J, Mantzoros CS. Leptin-dependent and leptin-independent pathways linking energy sufficiency with reproduction. Poster session presented at the Obesity Week Meeting; 2017 Oct 29-Nov 2; Washington DC.
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Shah A, Dodson WC, Kris-Etherton PM, Kunselman AR, Stetter CM, Gnatuk CL, Estes SJ, Allison KC, Sarwer DB, Sluss PM, Coutifaris C, Dokras A, Legro RS. Effects of Oral Contraception and Lifestyle Modification on Incretins and TGF-ß Superfamily Hormones in PCOS. J Clin Endocrinol Metab. 2021 Jan 1;106(1):108-119. doi:10.1210/clinem/dgaa682. PMID: 32968804; PMCID: PMC7765645
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Steiner AZ, Pritchard D, Stanczyk FZ, Kesner JS, Meadows JW, Herring AH, Baird DD. Association Between Biomarkers of Ovarian Reserve and Infertility Among Older Women of Reproductive Age. JAMA. 2017 Oct 10;318(14):1367-1376. doi: 10.1001/jama.2017.14588.
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Perakakis N, Upadhyay J, Ghaly W, Chen J, Chrysafi P, Anastasilakis AD, Mantzoros CS. Regulation of the activins-follistatins-inhibins axis by energy status: Impact on reproductive function. Metabolism. 2018 May 9. pii: S0026-0495(18)30120-3.
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Ting A, Savjani G, Kalra B, Kumar A, Stouffer R, Zelinski M. Dynamics of Growth and Differentiation Factor-9 and Inhibin B Production in Macaque Developing Follicles During 3-Dimensional Culture. Poster Presentation at ASRM 2017 Oct 28-Nov 1, San Antonio TX.
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Xingming Z, Qian Y. Relationship between inhibin B and different clinical phenotypes of polycystic ovary syndrome. Chinese Journal of Practical Gynecology and Obstetrics. 2017 eleven pages 1183-1188.
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