The Total Rat/Mouse IGFBP-5 enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of IGFBP-5 in mouse and rat samples.
| Catalog Number | |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 9.8-250 ng/mL |
| Limit of Detection | 0.40 ng/mL |
| Sample Size | 3-5 uL predilution |
| Sample Type | Serum |
| Assay Time | 2.5 hours |
| Species Reactivity | Murine Serum, Rattus Serum |
| Shelf Life | 24 months |
| Availability | Worldwide |
Insulin-like growth factor binding protein 5 (IGFBP-5) is part of the IGF-binding proteins that regulates IGF activity by binding to IGF ligands. The IGF axis consists of six high-affinity IGF-binding proteins (IGFBP 1-6). IGFBP-5 has 272 amino acids and weighs about 31 kDa. IGF axis is the principal endocrine system that regulates the differentiation, development, and maintenance of bone tissue. Dysfunction of this axis is associated with various skeletal pathologies such as growth disorders and abnormal bone structure. (1,2)
Pregnancy-Associated Plasma Protein-A2 (PAPP-A2) plays a major role in bone cell physiology as well. PAPP-A2 is also known to proteolyze IGFBP-5. Certain mutation in the PAPP-A2 protein inhibits its ability to proteolyze IGFBP-5. Consequently, affected individuals show microcephaly, mild BMD effects and thin long bones. Thus, IGFBP-5 plays an important role in osteoblast differentiation and bone tissue metabolism which remains to be further expounded upon. (1,2)
References:
1. Beattie et al. Insulin- like Growth Factor-Binding Protein Action in Bone Tissue: A Key Role for Pregnancy- Associated Plasma Protein-A. Front. Endocrinol., 2018
2. Duan, Cunming, and John B Allard. “Insulin-Like Growth Factor Binding Protein-5 in Physiology and Disease.” Frontiers in endocrinology vol. 11 100. 3 Mar. 2020, doi:10.3389/fendo.2020.00100