The Glicentin ELISA immunoassay kit uses highly characterized antibody pairs with precise antibody binding locations to eliminate cross-reactivity.
| Catalog Number | |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 6, 33.3-1666 pg/mL |
| Limit of Detection | 4.17 pg/mL |
| Sample Size | 25 µL |
| Sample Type | Plasma |
| Assay Time | 2.5 hours |
| Shelf Life | 24 months |
| Species Reactivity | Human, Bovine Serum, Canine Serum, Caprine Serum, Ovine Serum |
| Availability | Worldwide |
Glicentin is a 69 amino acid N-terminal peptide derived from proglucagon (PG 1-69) by posttranslational processing in the intestine by intestinal L-cells.Glicentin can further be processed into oxyntomodulin, corresponding to proglucagon residues no 33-69. These peptides are released simultaneously upon stimulation. Glicentin is a pro-glucagon derived gut peptide that is released in the gut in response to feed intake. It is present in high levels in thecirculation after stimulation and is often a problem when measuring glucagonbecause of its shared sequence.
Glicentin ELISA AL-185
D’Alessio D, Kumar A, Kalra B, Mistry S, Tong J. Novel ELISA Assays Demonstrate Specificity of Islet and Intestinal Processing of Proglucagon. Volume 4, Issue Supplement_1, April May 2020, SUN-LB124, https://doi.org/10
All Products Cited: GLP-1 ELISA AL-172; Glucagon ELISA AL-157; Oxyntomodulin ELISA AL-139; Glicentin ELISA AL-185
Kim S, Abbasi F, Nachmanoff C, Kumar A, Kalra B, Savjani G, Mantzoros C. Effect of Liraglutide Treatment on Proglucagon-Derived Peptides. JESOCI, Volume 4, Abstract Supplement, 2020
All Products Cited: GLP-1 ELISA AL-172; GLP-2 ELISA AL-174; Glucagon ELISA AL-157; Oxyntomodulin ELISA AL-139; Glicentin ELISA AL-185; MPGF ELISA AL-175
Perakakis N, Kalra B, Angelidi AM, Kumar A, Gavrieli A, MYannakoulia M, Mantzoros CS. Methods paper: Performance characteristics of novel assays for circulating levels of proglucagon-derived peptides and validation in a placebo controlled cross-over randomized clinical trial. Metabolism, Volume 129, 2022, 155157, ISSN 0026-0495, https://doi.org/10.1016/j.metabol.2022.155157.
All Products Cited: GLP-1 ELISA AL-172; GLP-2 ELISA AL-174; Oxyntomodulin ELISA AL-139; Glicentin ELISA AL-185; Glucagon ELISA AL-157; MPGF ELISA AL-175; C-Peptide of Insulin ELISA AL-151
Stefanakis K, Kokkinos A, Argyrakopoulou G, et al. Circulating levels of proglucagon derived peptides are differentially regulated by the glucagon like peptide-1 agonist liraglutide and the centrally acting naltrexone/bupropion and can predict future weight loss and metabolic improvements: A 6-month long interventional study. Diabetes Obes Metab. 023;25(9):2561‐2574. doi:10.1111/dom.15141
All Products Cited: C-Peptide of Insulin ELISA AL-151; Glicentin ELISA AL-185; GLP-1 ELISA AL-172; GLP-2 ELISA AL-174; Glucagon ELISA AL-157; Proglucagon ELISA AL-1019; MPGF ELISA AL-175; Oxyntomodulin AL-139
Yanni AE, Kokkinos A, Binou P, Papaioannou V, Halabalaki M, Konstantopoulos P, Simati S, Karathanos VT. Postprandial glucose and gastrointestinal hormone responses of healthy subjects to wheat biscuits enriched with L-arginine or branched-chain amino acids of plant origin. Nutrients. 2022;14(4381):1-17. doi:10.3390/nu14204381.
All Products Cited: Glicentin ELISA AL-185