The GDF-9 enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of GDF-9 in human serum and other biological fluids.
| Catalog Number | |
|---|---|
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Packaging | 96 well microtiter |
| Sample Size | 50 uL |
| Sample Type | Serum |
| Dynamic Range | 6, 48-5800 pg/mL |
| Limit of Detection | 3 pg/mL |
| Assay Time | 4.5 hours |
| Species Reactivity | Human, Bovine Serum, Canine Serum, Caprine Serum, Ovine Serum, Porcine Serum |
| Storage | Store at 2 to 8°C until expiration date. |
| Availability | Worldwide |
BMP-15 and GDF-9 are synthesized as 249-295 amino acid N-terminal prodomains and 125-139 amino acid mature domains. The mature domains of GDF-9 and BMP-15 form 40 kDa and 34 kDa homodimers respectively and 37 kDa heterodimer 1,2. Growth Differentiation Factor 9 (GDF9) and Bone Morphogenic Protein 9 (BMP15) are oocyte secreted factors and play key roles in promoting follicle growth, maturation and oocyte competence 1,3-5. However, the action of BMP15 and GDF9 varied with respect to the species of origin and the stages of follicle development. Oocyte competence is an intrinsic developmental potential that allows a mature oocyte to become fertilized and develop to an embryo. Oocyte competence is achieved by long course of molecular, biochemical and morphological changes4. Recent study suggests AMH production is regulated by oocyte-secreted factors in primary human cumulus cells and the combination of GDF9 + BMP15 potently stimulates AMH expression6. Altered level and activity of GDF9 is implicated in premature ovarian failure and polycystic ovarian syndrome, implicating GDF9 in the etiology of these conditions 7-9.
References:
1. Proc Natl Acad Sci U S A. 2013 Feb;110(8): E776-85.
2. Endocrinology. 2012 Mar;153(3):1509-18.
3. J Biol Chem. 2015 Sep;290(39):24007-20.
4. Reproduction. 2005 Apr;129(4):473-80.
5. Reproduction. 2017 Dec;154(6):745-753
6. Dev Biol. 2006 Aug 15;296(2):514-21.
7. J Assist Reprod Genet. 2012 Oct;29(10):1057-65.
8. J Clin Endocrinol Metab. 2016 Dec;101(12):4541-4550.
9. J Clin Endocrinol Metab. 2014 Apr;99(4): E615-24.
GDF-9 ELISA AL-176
Cadenas J, Pors SE, Kumar A, Kalra B, Kristensen SG, Andersen CY, Mamsen LS. Concentrations of oocyte secreted GDF9 and BMP15 decrease with MII transition during human IVM. Reprod Biol Endocrinol. 2022;20(126):1-10. doi:10.1186/s12958-022-01000-6(917).
All Products Cited: GDF-9 antibodies AB-325-x; BMP-15 antibodies AB-324-x; GDF-9 ELISA AL-176; BMP-15 ELISA AL-179; GDF-9/BMP-15 Complex ELISA AL-181
J Cadenas, L C Poulsen, D Nikiforov, M L Grøndahl, A Kumar, K Bahnu, A L M Englund, J Malm, G Marko-Varga, I Pla, A Sanchez, S E Pors, C Yding Andersen, Regulation of human oocyte maturation in vivo during the final maturation of follicles, Human Reproduction, Volume 38, Issue 4, April 2023, Pages 686–700, https://doi.org/10.1093/humrep/dead024
All Products Cited: Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Activin B ELISA AL-150; Activin AB ELISA AL-153; Activin A ELISA AL-110; GDF-9 ELISA AL-176; GDF-9/BMP-15 Complex ELISA AL-181; BMP-15 ELISA AL-179
Kalra B, Chowdavarapu K, Patel AS, Mistry S, Budhavarapu V, Savjani G, Pasternack A, Laitinen MP, Hyvonen M, Ritvos O, Kumar A. Development of Novel ELISAs for Human GDF-9 and BMP-15 homodimers and the GDF-9:BMP-15 heterodimer. Poster presented at 99th Endocrine Society Annual Meeting; 2017 April 1-4; Orlando, FL
All Products Cited: BMP-15 ELISA AL-179; GDF-9 ELISA AL-176
Kristensen SG, Kumar A, Kalra B, Pors SE, Bøtkjær JA, Mamsen LS, Colmorn LB, Fedder J, Ernst E, Owens LA, Hardy K, Franks S, Andersen CY. Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO. J Clin Endocrinol Metab. 2019 Dec 1;104(12):6371-6384. doi: 10.1210/jc.2019-01094. PMID: 31287539.
All Products Cited: GDF-9 ELISA AL-176; AMH (pico) ELISA AL-124; Inhibin-A ELISA AL-123; Inhibin-B ELISA AL-107; Inhibin (Total) ELISA AL-134; Activin-A ELISA AL-110; Activin-B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; FTSL-3 ELISA AL-152
Kristensen SG, Kumar A, Mamsen LS, Kalra B, Pors SE, Bøtkjær JA, Macklon KT, Fedder J, Ernst E, Hardy K, Franks S, Andersen CY. Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries. J Clin Endocrinol Metab. 2022 May 2:dgac272. doi: 10.1210/clinem/dgac272. Epub ahead of print. PMID: 35511085.
All Products Cited: GDF-9 ELISA AL-176; BMP-15 ELISA AL-179; GDF-9/BMP-15 Complex ELISA AL-181; AMH ELISA AL-105; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Inhibin (Total) ELISA AL-134; Activin B ELISA AL-110; Activin AB ELISA AL-153; Follistatin ELISA AL-117
Kristensen SG. Altered Levels of TGF-Beta Superfamily Growth Factors in Small Antral Follicle Fluids from Polycystic Ovaries. Poster session presented at the International Congress of Endocrinology, 2018 Dec 1-4, Cape Town South Africa.
All Products Cited: GDF-9 ELISA AL-176; AMH ELISA AL-105; Activin A ELISA AL-110; Activin B ELISA AL-150; Activin AB ELISA Al-153; Follistatin ELISA AL-117
Ting A, Savjani G, Kalra B, Kumar A, Stouffer R, Zelinski M. Dynamics of Growth and Differentiation Factor-9 and Inhibin B Production in Macaque Developing Follicles During 3-Dimensional Culture. Poster Presentation at ASRM 2017 Oct 28-Nov 1, San Antonio TX.
All Products Cited: GDF-9 ELISA AL-176; Inhibin B ELISA AL-107