The BMP-15 Enzyme-Linked Immunosorbent Assay (ELISA) kit provides materials for the quantitative measurement of BMP-15 in follicular fluid.
| Catalog Number | |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Limit of Detection | 29 pg/mL |
| Dynamic Range | 6, 62-6690 ng/mL |
| Sample Size | 50 µL |
| Sample Type | Follicular Fluid |
| Assay Time | 4.5 hours |
| Shelf Life | 24 months |
| Species Reactivity | Bovine (Follicular Fluid), Human, Bovine Serum, Canine Serum, Caprine Serum, Ovine Serum, Porcine Serum |
| Availability | Worldwide |
Growth Differentiation Factor 9 (GDF-9) and its closest paralog Bone Morphogenetic Protein 15 (BMP15; also known as GDF-9B) are oocyte-specific growth factors and distinguish themselves from granulosa cell-derived markers of ovarian function (such as AMH, inhibin A, inhibin B, E2) and thus may provide a more direct assessment of oocyte function competence. The mature peptide portions of GDF-9 and BMP-15 can form non-covalent bioactive homo- and heterodimers, but no methods have been available for measuring these different forms in biological fluids. BMP-15 and GDF-9 are synthesized as precursors with 249-295 amino acid N-terminal pro-peptides and 125-139 amino acid mature domains. GDF-9 and BMP-15 form 40 kDa and 34 kDa homodimers and 37 kDa heterodimers. Recent evidence shows that the GDF-9:BMP-15 is a highly active GDF-9-like super agonist being up to 1000-fold more potent than GDF-9 itself. GDF-9:BMP-15 appears to be the biologically most relevant form of the dimers.
BMP-15 ELISA AL-179
Cadenas J, Pors SE, Kumar A, Kalra B, Kristensen SG, Andersen CY, Mamsen LS. Concentrations of oocyte secreted GDF9 and BMP15 decrease with MII transition during human IVM. Reprod Biol Endocrinol. 2022;20(126):1-10. doi:10.1186/s12958-022-01000-6(917).
All Products Cited: GDF-9 antibodies AB-325-x; BMP-15 antibodies AB-324-x; GDF-9 ELISA AL-176; BMP-15 ELISA AL-179; GDF-9/BMP-15 Complex ELISA AL-181
J Cadenas, L C Poulsen, D Nikiforov, M L Grøndahl, A Kumar, K Bahnu, A L M Englund, J Malm, G Marko-Varga, I Pla, A Sanchez, S E Pors, C Yding Andersen, Regulation of human oocyte maturation in vivo during the final maturation of follicles, Human Reproduction, Volume 38, Issue 4, April 2023, Pages 686–700, https://doi.org/10.1093/humrep/dead024
All Products Cited: Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Activin B ELISA AL-150; Activin AB ELISA AL-153; Activin A ELISA AL-110; GDF-9 ELISA AL-176; GDF-9/BMP-15 Complex ELISA AL-181; BMP-15 ELISA AL-179
Kalra B, Chowdavarapu K, Patel AS, Mistry S, Budhavarapu V, Savjani G, Pasternack A, Laitinen MP, Hyvonen M, Ritvos O, Kumar A. Development of Novel ELISAs for Human GDF-9 and BMP-15 homodimers and the GDF-9:BMP-15 heterodimer. Poster presented at 99th Endocrine Society Annual Meeting; 2017 April 1-4; Orlando, FL
All Products Cited: BMP-15 ELISA AL-179; GDF-9 ELISA AL-176
Kristensen SG, Kumar A, Mamsen LS, Kalra B, Pors SE, Bøtkjær JA, Macklon KT, Fedder J, Ernst E, Hardy K, Franks S, Andersen CY. Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries. J Clin Endocrinol Metab. 2022 May 2:dgac272. doi: 10.1210/clinem/dgac272. Epub ahead of print. PMID: 35511085.
All Products Cited: GDF-9 ELISA AL-176; BMP-15 ELISA AL-179; GDF-9/BMP-15 Complex ELISA AL-181; AMH ELISA AL-105; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Inhibin (Total) ELISA AL-134; Activin B ELISA AL-110; Activin AB ELISA AL-153; Follistatin ELISA AL-117