The BMP-15 Enzyme-Linked Immunosorbent Assay (ELISA) kit provides materials for the quantitative measurement of BMP-15 in follicular fluid.
| Catalog Number | |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Limit of Detection | 29 pg/mL |
| Dynamic Range | 6, 62-6690 ng/mL |
| Sample Size | 50 µL |
| Sample Type | Follicular Fluid |
| Assay Time | 4.5 hours |
| Shelf Life | 24 months |
| Species Reactivity | Bovine (Follicular Fluid), Human, Bovine Serum, Canine Serum, Caprine Serum, Ovine Serum, Porcine Serum |
| Availability | Worldwide |
Growth Differentiation Factor 9 (GDF-9) and its closest paralog Bone Morphogenetic Protein 15 (BMP15; also known as GDF-9B) are oocyte-specific growth factors and distinguish themselves from granulosa cell-derived markers of ovarian function (such as AMH, inhibin A, inhibin B, E2) and thus may provide a more direct assessment of oocyte function competence. The mature peptide portions of GDF-9 and BMP-15 can form non-covalent bioactive homo- and heterodimers, but no methods have been available for measuring these different forms in biological fluids. BMP-15 and GDF-9 are synthesized as precursors with 249-295 amino acid N-terminal pro-peptides and 125-139 amino acid mature domains. GDF-9 and BMP-15 form 40 kDa and 34 kDa homodimers and 37 kDa heterodimers. Recent evidence shows that the GDF-9:BMP-15 is a highly active GDF-9-like super agonist being up to 1000-fold more potent than GDF-9 itself. GDF-9:BMP-15 appears to be the biologically most relevant form of the dimers.
BMP-15
BMP-15 ELISA AL-179
Kalra B, Chowdavarapu K, Patel AS, Mistry S, Budhavarapu V, Savjani G, Pasternack A, Laitinen MP, Hyvonen M, Ritvos O, Kumar A. Development of Novel ELISAs for Human GDF-9 and BMP-15 homodimers and the GDF-9:BMP-15 heterodimer. Poster presented at 99th Endocrine Society Annual Meeting; 2017 April 1-4; Orlando, FL
All Products Cited: BMP-15 ELISA AL-179; GDF-9 ELISA AL-176
Kristensen SG, Kumar A, Mamsen LS, Kalra B, Pors SE, Bøtkjær JA, Macklon KT, Fedder J, Ernst E, Hardy K, Franks S, Andersen CY. Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries. J Clin Endocrinol Metab. 2022 May 2:dgac272. doi: 10.1210/clinem/dgac272. Epub ahead of
print. PMID: 35511085.
All Products Cited: GDF-9 ELISA AL-176; BMP-15 ELISA AL-179; GDF-9/BMP-15 Complex ELISA AL-181; AMH ELISA AL-105; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Total Inhibin ELISA AL-134; Activin B ELISA AL-110; Activin AB ELISA AL-153; Follistatin ELISA AL-117