The Activin B enzyme linked immunuosorbent assay (ELISA) kit provides materials for the quantitative measurement of Activin B in human serum, lithium heparin plasma and other biological fluids.
| Catalog Numbers | |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 6, 12-2600 pg/mL |
| Limit of Detection | 4.35 pg/mL |
| Sample Size | 25 µL |
| Sample Type | Plasma, Serum |
| Assay Time | 3.5 hours |
| Shelf Life | 24 months |
| Species Reactivity | Human, Bovine Serum, Canine Serum, Canine Testicular Fluid, Caprine Serum, Equine Cyst Fluid, Feline Serum, Ovine Serum, Porcine Serum, Squirrel Monkey Serum |
| Availability | Worldwide |
| Analyte | Activin B |
Activins, like all members of the transforming growth factor-beta superfamily, are synthesized as large pro-hormones with N-terminal pro- and C-terminal mature domains. Studies have shown that the prodomains template the dimerization of pro-activin forms, that are subsequently processed to yield pro/mature fragments. The prodomains, although important for folding and extracellular localization, must be removed prior to activity. Activin forms retaining the prodomain are biologically inactive.
Activin B, like certain other members of the TGF-β family, signals through the ActRII receptor (Activin Receptor type II). Human Activin B is a 25.6 kDa disulfide-linked homodimer consisting of two βB chains, each containing 115 amino acid residues.
Activin B exhibits a wide range of biological activities, including regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands.
Activin B ELISA AL-150
Anastasilakis AD, Polyzos SA, Rodopaios NE, Makras P, Kumar A, Kalra B, Mantzoros CS. Activins, follistatins and inhibins in postmenopausal osteoporosis: A proof of concept, case-control study. Metabolism. 2022 Dec 29:155397. doi: 10.1016/j.metabol.2022.155397. Epub ahead of print. PMID: 36587801.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; Inhibin B ELISA AL-107; Inhibin A ELISA AL-123; Follistatin-Like 3 ELISA AL-152
Calvert ME, Kalra B, Patel A, Kumar A, Shaw ND. Serum and urine profiles of TGF-β superfamily members in reproductive aged women. Clin Chim Acta. 2022 Jan 1;524:96-100. doi: 10.1016/j.cca.2021.12.002. Epub 2021 Dec 5. PMID: 34875272; PMCID: PMC8740174.
All Products Cited: Inhibin A (pico) ELISA AL-184; Inhibin B ELISA AL-107; Inhibin (Total) ELISA AL-134; AMH (PCOCheck) ELISA AL-196; Activin A ELISA AL-110; Activin B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; GDF-9/BMP-15 Complex ELISA AL-181; GDF-15 (Total) ELISA AL-1014; GDF-15 (H-Specific) ELISA AL-1018
Guignabert C, Savale L, Boucly A, Thuillet R, Tu L, Ottaviani M, Rhodes CJ, De Groote P, Prévot G, Bergot E, Bourdin A, Howard LS, Fadel E, Beurnier A, Roche A, Jevnikar M, Jaïs X, Montani D, Wilkins MR, Sitbon O, Humbert M. Serum and Pulmonary Expression Profiles of the Activin Signaling System in Pulmonary Arterial Hypertension. Circulation. 2023 Jun 13;147(24):1809-1822. doi:10.1161/CIRCULATIONAHA.122.061501. Epub 2023 Apr 25. PMID: 37096577.
All Products Cited: Activin B ELISA AL-150; Follistatin-Like 3 ELISA AL-152; Inhibin (Total) ELISA AL-134
Houston BJ, O’Connor AE, Wang D, Goodchild G, Merriner DJ, Luan H, Conrad DF, Nagirnaja L, Aston KI, Kliesch S, Wyrwoll MJ, Friedrich C, Tüttelmann F, Harrison C, O’Bryan MK, Walton K. Human INHBB Gene Variant (c.1079T>C:p.Met360Thr) Alters Testis Germ Cell Content, but Does Not Impact Fertility in Mice. Endocrinology. 2022 Mar 1;163(3):bqab269. doi: 10.1210/endocr/bqab269. PMID: 35022746.
All Products Cited: Activin B ELISA AL-150
J Cadenas, L C Poulsen, D Nikiforov, M L Grøndahl, A Kumar, K Bahnu, A L M Englund, J Malm, G Marko-Varga, I Pla, A Sanchez, S E Pors, C Yding Andersen, Regulation of human oocyte maturation in vivo during the final maturation of follicles, Human Reproduction, Volume 38, Issue 4, April 2023, Pages 686–700, https://doi.org/10.1093/humrep/dead024
All Products Cited: Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Activin B ELISA AL-150; Activin AB ELISA AL-153; Activin A ELISA AL-110; GDF-9 ELISA AL-176; GDF-9/BMP-15 Complex ELISA AL-181; BMP-15 ELISA AL-179
Kalra B, Patel A, Kommareddy V, Ganapathy K, Mistry S, Budhavarapu V, Savjani G, Pasternack A, Ritvos O, Hyvonen M, Kumar A . Development of Novel Specific ELISAs for all Inhibins, Activins and their Binding Proteins FST and FSTL3. Poster presented at 98th Annual Endocrine Society Meeting; 2016 Apr 1-3; Boston, MA.
All Products Cited: Activin A ELISA AL-110; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Activin B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; Follistatin-Like 3 ELISA AL-152
Kristensen SG, Kumar A, Kalra B, Pors SE, Bøtkjær JA, Mamsen LS, Colmorn LB, Fedder J, Ernst E, Owens LA, Hardy K, Franks S, Andersen CY. Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO. J Clin Endocrinol Metab. 2019 Dec 1;104(12):6371-6384. doi: 10.1210/jc.2019-01094. PMID: 31287539.
All Products Cited: GDF-9 ELISA AL-179; AMH (pico) ELISA AL-124; Inhibin-A ELISA AL-123; Inhibin-B ELISA AL-107; Inhibin (Total) ELISA AL-134; Activin-A ELISA AL-110; Activin-B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; FTSL-3 ELISA AL-152
Kristensen SG, Kumar A, Mamsen LS, Kalra B, Pors SE, Bøtkjær JA, Macklon KT, Fedder J, Ernst E, Hardy K, Franks S, Andersen CY. Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries. J Clin Endocrinol Metab. 2022 May 2:dgac272. doi: 10.1210/clinem/dgac272. Epub ahead of print. PMID: 35511085.
All Products Cited: GDF-9 ELISA AL-176; BMP-15 ELISA AL-179; GDF-9/BMP-15 Complex ELISA AL-181; AMH ELISA AL-105; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Inhibin (Total) ELISA AL-134; Activin B ELISA AL-110; Activin AB ELISA AL-153; Follistatin ELISA AL-117
Kristensen SG. Altered Levels of TGF-Beta Superfamily Growth Factors in Small Antral Follicle Fluids from Polycystic Ovaries. Poster session presented at the International Congress of Endocrinology, 2018 Dec 1-4, Cape Town South Africa.
All Products Cited: GDF-9 ELISA AL-176; AMH ELISA AL-105; Activin A ELISA AL-110; Activin B ELISA AL-150; Activin AB ELISA Al-153; Follistatin ELISA AL-117
Paajanen J, Ilonen I, Lauri H, Järvinen T, Sutinen E, Ollila H, Rouvinen E, Lemström K, Räsänen J, Ritvos O, Koli K, Myllärniemi M. Elevated Circulating Activin A Levels in Patients With Malignant Pleural Mesothelioma Are Related to Cancer Cachexia and Reduced Response to Platinum-based Chemotherapy. Clin Lung Cancer. 2019 Oct 25. pii: S1525-7304(19)30287-6. doi: 10.1016/j.cllc.2019.10.013. [Epub ahead of print]
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117; Follistatin-Like 3 ELISA AL-152
Panagiotou G, Ghaly W, Upadhyay J, Pazaitou-Panayiotou K, Mantzoros CS. Serum Follistatin Is Increased in Thyroid Cancer and Is Associated With Adverse Tumor Characteristics in Humans. J Clin Endocrinol Metab. 2021 Apr 23;106(5):e2137-e2150. doi: 10.1210/clinem/dgab041. PMID: 33493282.
All Products Cited: Follistatin ELISA AL-117; Follistatin-Like 3 ELISA AL-152; Activin A ELISA AL-110; Activin B ELISA AL-150; Stanniocalcin 2 ELISA AL-143; IGF-I (Free) ELISA AL-122
Panagiotou G, Papakonstantinou E, Vagionas A, Polyzos SA, Mantzoros CS. Serum Levels of Activins, Follistatins, and Growth Factors in Neoplasms of the Breast: A Case-Control Study. J Clin Endocrinol Metab. 2019 Feb 1;104(2):349-358.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117; Follistatin-Like 3 ELISA AL-152; IGF-I (Total) ELISA AL-121; IGFBP-4 (Total) ELISA AL-126; IGFBP-4 (Intact) ELISA AL-128
Peradze N, Farr OM, Perakakis N, Lázaro I, Sala-Vila A, Mantzoros CS. Short-term treatment with high dose liraglutide improves lipid and lipoprotein profle and changes hormonal mediators of lipid metabolism in obese patients with no overt type 2 diabetes mellitus: a randomized, placebo-controlled, cross-over, double-…. Peradze et al. Cardiovasc Diabetol (2019) 18:141 https://doi.org/10.1186/s12933-019-0945-7
All Products Cited: Activin A ELISA AL-110; Activin AB ELISA AL-153; Activin B ELISA AL-150; Follistatin ELISA AL-117;
Follistatin-Like 3 ELISA AL-152
Perakakis N, Chen J, Ghaly W, Vamvini MT, Mantzoros CS. Effects of oral and intravenous lipid administration on hormones affecting energy balance. Poster session presented at the Obesity Week Meeting; 2017 Oct 29-Nov 2; Washington DC.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin-Like 3 ELISA AL-152; Oxyntomodulin ELISA AL-139
Perakakis N, Mougios V, Fatouros I, Siopi A, Draganidis D, Peradze N, Ghaly W, Mantzoros CS. Physiology of Activins/Follistatins: Associations With Metabolic and Anthropometric Variables and Response to Exercise. J Clin Endocrinol Metab. 2018 Oct 1;103(10):3890-3899.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117; Follistatin-like 3 ELISA AL-152
Perakakis N, Upadhyay J, Ghaly W, Chen J, Chrysafi P, Anastasilakis AD, Mantzoros CS. Regulation of the activins-follistatins-inhibins axis by energy status: Impact on reproductive function. Metabolism. 2018 May 9. pii: S0026-0495(18)30120-3.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin-Like 3 ELISA AL-152; Inhibin B ELISA AL-107; AMH ELISA AL-105
Perakakis N, Upadhyay J, Ghaly W, Chen J, Mantzoros CS. Leptin-dependent and leptin-independent pathways linking energy sufficiency with reproduction. Poster session presented at the Obesity Week Meeting; 2017 Oct 29-Nov 2; Washington DC.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin-Like 3 ELISA AL-152; Inhibin B ELISA AL-107; AMH ELISA AL-105
Polyzos SA, Perakakis N, Boutari C, Kountouras J, Ghaly W, Anastasilakis AD, Karagiannis A, Mantzoros CS. Targeted Analysis of Three Hormonal Systems Identifies Molecules Associated with the Presence and Severity of NAFLD. J Clin Endocrinol Metab. 2020 Mar 1;105(3):e390–400. doi: 10.1210/clinem/dgz172. PMID: 31690932; PMCID: PMC7112980.
All Products Cited: GLP-1 ELISA AL-172; GLP-2 ELISA AL-174; Glucagon ELISA AL-157; MPGF ELISA AL-175; Oxyntomodulin ELISA AL-139; Follistatin-Like 3 ELISA AL-152; Activin B ELISA AL-150; IGF-I (Total) ELISA AL-121; IGFBP-3 (Total) ELISA AL-120; IGFBP-3 (Intact) ELISA AL-149; IGFBP-4 (Total) ELISA AL-126; IGFBP-4 (Intact) ELISA AL-128; PAPP-A (pico) ELISA AL-101
Richani D, Constance K, Lien S, Agapiou D, Stocker WA, Hedger MP, Ledger WL, Thompson JG, Robertson DM, Mottershead DG, Walton KL, Harrison CA, Gilchrist RB. Cumulin and FSH Cooperate to Regulate Inhibin B and Activin B Production by Human Granulosa-Lutein Cells In Vitro. Endocrinology. 2019 Apr 1;160(4):853-862.
All Products Cited: Activin B ELISA AL-150
Synolaki E, Papadopoulos V, Divolis G, Tsahouridou O, Gavriilidis E, Loli G, Gavriil A, Tsigalou C, Tziolos NR, Sertaridou E, Kalra B, Kumar A, Rafailidis P, Pasternack A, Boumpas DT, Germanidis G, Ritvos O, Metallidis S, Skendros P, Sideras P. The Activin/Follistatin-axis is severely deregulated in COVID-19 and independently associated with in-hospital mortality. J Infect Dis. 2021 Feb 24:jiab108. doi: 10.1093/infdis/jiab108. Epub ahead of print. PMID: 33625513; PMCID: PMC7928794
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117
Valenzuela-Vallejo L, Chrysafi P, Agraz M, Connelly M, Papatheodoridis G, Verrastro O, Mingrone G, Mantzoros CS. GROWTH DIFFERENTIATION FACTOR 15 (GDF-15) LEVELS IS ASSOCIATED WITH THE PRESENCE AND SEVERITY OF NAFLD. Nutr Diabetes. 2022 Feb 22;12(1):9. doi: 10.1038/s41387-022-00187-2. PMID: 35194014; PMCID: PMC8863897
All Products Cited: GDF-15 (H-Specific) ELISA AL-1018; Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117; Follistatin-Like 3 ELISA AL-152; IGF-I (Free) AL-122
Wang Y, Hamang M, Culver A, Jiang H, Yanum J, Garcia V, Lee J, White E, Kusumanchi P, Chalasani N, Liangpunsakul S, Yaden BC, Dai G. Activin B promotes the initiation and progression of liver fibrosis. Hepatol Commun. 2022 Oct;6(10):2812-2826. doi: 10.1002/hep4.2037.
All Products Cited: Activin B ELISA AL-150; Activin B Antibodies