The Activin A enzyme linked immunuosorbent assay (ELISA) kit provides materials for the quantitative measurement of Activin A in human serum and other biological fluids.
| Catalog Numbers | AL-110 (RUO) Instructions For Use |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 6, 0.1-10 ng/mL |
| Limit of Detection | 0.065 ng/mL |
| Sample Size | 25 µL |
| Sample Type | Serum |
| Assay Time | 3.5 hours |
| Shelf Life | 24 months |
| Species Reactivity | Human, Bovine Serum, Canine Serum, Canine Testicular Fluid, Caprine Serum, Equine Cyst Fluid, Equine Serum, Feline Serum, Murine Serum, Ovine Serum, Porcine Serum, Squirrel Monkey Serum, Vervet Monkey Serum |
| Availability | Worldwide |
Activin-A is a TGF-beta family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. The biological activities of Activin-A can be neutralized by inhibins and by the diffusible TGF-beta antagonist, Follistatin. Human Activin-A is a 26 kDa disulfide-linked homodimer of two beta A chains, each containing 116 amino acid residues. Elevated levels of Activin-A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively.
References:
1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
2. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.
4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
Activin A ELISA AL-110
Legro R, Dodson W, Estes S, Kunselman A, Coutifaris C, Dokras A. Effect of Weight Loss and Hormonal Suppression Alone and in Combination on Bioactive Gut and Ovarian Peptide Hormones in Women with Polycystic Ovary Syndrome. Journal of the Endocrine Society, Volume 3, Issue Supplement_1, April-May 2019, MON-233,
https://doi.org/10.1210/js.2019-MON-233
All Products Cited: Activin A ELISA AL-110; Follistatin ELISA AL-117; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; IGF-I ELISA AL-121; IGFBP-2 ELISA AL-140; Oxyntomodulin ELISA AL-139
Metallinou D, Karampas G, Lazarou E, Iacovidou N, Pervanidou P, Lykeridou K, Mastorakos G, Rizos D. Serum Activin A as Brain Injury Biomarker in the First Three Days of Life. A Prospective Case—Control Longitudinal Study in Human Premature Neonates. Brain Sci. 2021, 11, 1243. https://doi.org/10.3390/brainsci11091243
All Products Cited: Activin A ELISA AL-110
Paajanen J, Ilonen I, Lauri H, Järvinen T, Sutinen E, Ollila H, Rouvinen E, Lemström K, Räsänen J, Ritvos O, Koli K, Myllärniemi M. Elevated Circulating Activin A Levels in Patients With Malignant Pleural Mesothelioma Are Related to Cancer Cachexia and Reduced Response to Platinum-based Chemotherapy. Clin Lung Cancer. 2019 Oct
25. pii: S1525-7304(19)30287-6. doi: 10.1016/j.cllc.2019.10.013. [Epub ahead of print]
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117; Follistatin Like 3 ELISA AL-152
Shahul S, Ramadan H, Nizamuddin J, Mueller A, Patel V, Dreixler J, Tung A, Lang RM, Weinert L, Nasim R, Chinthala S, Rana S. Activin A and Late Postpartum Cardiac Dysfunction Among Women With Hypertensive Disorders of Pregnancy. Hypertension. 2018 Jul;72(1):188-193. doi: 10.1161/HYPERTENSIONAHA.118.10888. Epub 2018
May 29. PMID: 29844146.
All Products Cited: Activin A ELISA AL-110
Panagiotou G, Ghaly W, Upadhyay J, Pazaitou-Panayiotou K, Mantzoros CS. Serum Follistatin Is Increased in Thyroid Cancer and Is Associated With Adverse Tumor Characteristics in Humans. J Clin Endocrinol Metab. 2021 Apr 23;106(5):e2137-e2150. doi: 10.1210/clinem/dgab041. PMID: 33493282.
All Products Cited: Follistatin ELISA AL-117; Follistatin Like 3 ELISA AL-152; Activin A ELISA AL-110; Activin B ELISA AL-150; Stanniocalcin 2 ELISA AL-143; Free IGF-I ELISA AL-122
Kim SY, Ebbert K, Cordeiro MH, Romero MM, Whelan KA, Suarez AA, Woodruff TK, Kurita T. Constitutive Activation of PI3K in Oocyte Induces Ovarian Granulosa Cell Tumors. Cancer Res. 2016 Jul 1;76(13):3851-61
All Products Cited: Activin A ELISA AL-110; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107
Kristensen SG, Kumar A, Kalra B, Pors SE, Bøtkjær JA, Mamsen LS, Colmorn LB, Fedder J, Ernst E, Owens LA, Hardy K, Franks S, Andersen CY. Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO. J Clin Endocrinol Metab. 2019 Dec 1;104(12):6371-6384. doi:
10.1210/jc.2019-01094. PMID: 31287539.
All Products Cited: GDF-9 ELISA AL-179; picoAMH ELISA AL-124; Inhibin-A ELISA AL-123; Inhibin-B ELISA AL-107; Inhibin (Total) ELISA AL-134; Activin-A ELISA AL-110; Activin-B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; FTSL-3 ELISA AL-152
Kalra B, Patel A, Kommareddy V, Ganapathy K, Mistry S, Budhavarapu V, Savjani G, Pasternack A, Ritvos O, Hyvonen M, Kumar A. Development of Novel Specific ELISAs for all Inhibins, Activins and their Binding Proteins FST and FSTL3. Poster presented at 98th Annual Endocrine Society Meeting; 2016 Apr 1-3; Boston, MA.
All Products Cited: Activin A ELISA AL-110; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; Activin B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; Follistatin Like 3 ELISA AL-152
Peradze N, Farr OM, Perakakis N, Lázaro I, Sala-Vila A, Mantzoros CS. Short-term treatment with high dose liraglutide improves lipid and lipoprotein profle and changes hormonal mediators of lipid metabolism in obese patients with no overt type 2 diabetes mellitus: a randomized, placebo-controlled, cross-over, double-…. Peradze et al.
Cardiovasc Diabetol (2019) 18:141 https://doi.org/10.1186/s12933-019-0945-7
All Products Cited: Activin A ELISA AL-110; Activin AB ELISA AL-153; Activin B ELISA AL-150; Follistatin ELISA AL-117; Follistatin-Like 3 ELISA AL-152
Bakrania BA, Palei AC, Bhattarai U, Chen Y, Granger JP, Shahul S. Sustained Elevated Circulating Activin A Impairs Global Longitudinal Strain in Pregnant Rats: A Potential Mechanism for Preeclampsia-Related Cardiac Dysfunction. Cells. 2022 Feb 21;11(4):742. doi: 10.3390/cells11040742. PMID: 35203391; PMCID: PMC8870359.
All Products Cited: Activin A ELISA AL-110; Activin A Antigens
Palin NK, Savikko J, Pasternack A, Rintala JM, Kalra B, Mistry S, Kumar A, Roth MP, Helin H, Ritvos O. Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study. Transpl Int. 2017 Jan;30(1):96-107. doi: 10.1111/tri.12876. Epub 2016 Nov 23. PMID: 27732750.
All Products Cited: Activin B (Mouse) ELISA AL-156; Activin A ELISA AL-110
deMartelly VA, Dreixler J, Tung A, Mueller A, Heimberger S, Fazal AA, Naseem H, Lang R, Kruse E, Yamat M, Granger JP, Bakrania BA, Rodriguez-Kovacs J, Rana S, Shahul S. Long-Term Postpartum Cardiac Function and Its Association With Preeclampsia. J Am Heart Assoc. 2021 Feb;10(5):e018526. doi: 10.1161/JAHA.120.018526
All Products Cited: Activin A ELISA AL-110; Follistatin ELISA AL-117
Naseem H, Dreixler J, Mueller A, Tung A, Dhir R, Chibber R, Fazal A, Granger JP, Bakrania BA, deMartelly V, Rana S, Shahul S. Antepartum Aspirin Administration Reduces Activin A and Cardiac Global Longitudinal Strain in Preeclamptic Women. J Am Heart Assoc. 2020 Jun 16;9(12):e015997. doi: 10.1161/JAHA.119.015997. Epub 2020 Jun
4. PMID: 32495688; PMCID: PMC7429043.
All Products Cited: Activin A ELISA AL-110; Follistatin ELISA AL-117, Follistatin-Like 3 ELISA AL-152
Kristensen SG. Altered Levels of TGF-Beta Superfamily Growth Factors in Small Antral Follicle Fluids from Polycystic Ovaries. Poster session presented at the International Congress of Endocrinology, 2018 Dec 1-4, Cape Town South Africa.
All Products Cited: GDF-9 ELISA AL-176; AMH ELISA AL-105; Activin A ELISA AL-110; Activin B ELISA AL-150; Activin AB ELISA Al-153; Follistatin ELISA AL-117
Perakakis N, Upadhyay J, Ghaly W, Chen J, Chrysafi P, Anastasilakis AD, Mantzoros CS. Regulation of the activins-follistatins-inhibins axis by energy status: Impact on reproductive function. Metabolism. 2018 May 9. pii: S0026-0495(18)30120-3.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin Like 3 ELISA AL-152; Inhibin B ELISA AL-107; AMH ELISA AL-105
Skory R, Xu Y, Shea L, Woodruff T. Engineering the ovarian cycle using in vitro follicle culture. Human Reproduction 2015 Mar 16 Epub ahead of print.
All Products Cited: Activin A ELISA AL-110
Perakakis N, Upadhyay J, Ghaly W, Chen J, Mantzoros CS. Leptin-dependent and leptin-independent pathways linking energy sufficiency with reproduction. Poster session presented at the Obesity Week Meeting; 2017 Oct 29-Nov 2; Washington DC.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin Like 3 ELISA AL-152; Inhibin B ELISA AL-107; AMH ELISA AL-105
Synolaki E, Papadopoulos V, Divolis G, Tsahouridou O, Gavriilidis E, Loli G, Gavriil A, Tsigalou C, Tziolos NR, Sertaridou E, Kalra B, Kumar A, Rafailidis P, Pasternack A, Boumpas DT, Germanidis G, Ritvos O, Metallidis S, Skendros P, Sideras P. The Activin/Follistatin-axis is severely deregulated in COVID-19 and independently associated with in-hospital mortality. J Infect Dis. 2021 Feb 24:jiab108. doi: 10.1093/infdis/jiab108. Epub ahead of print. PMID: 33625513; PMCID: PMC7928794
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117
Shah A, Dodson WC, Kris-Etherton PM, Kunselman AR, Stetter CM, Gnatuk CL, Estes SJ, Allison KC, Sarwer DB, Sluss PM, Coutifaris C, Dokras A, Legro RS. Effects of Oral Contraception and Lifestyle Modification on Incretins and TGF-ß Superfamily Hormones in PCOS. J Clin Endocrinol Metab. 2021 Jan 1;106(1):108-119. doi:10.1210/clinem/dgaa682. PMID: 32968804; PMCID: PMC7765645
All Products Cited: Activin A ELISA AL-110; Activin AB ELISA AL-153; Inhibin A ELISA AL-123; Inhibin B ELISA AL-107; AMH ELISA AL-105, IGF-I ELISA AL-121, IGFBP-2 ELISA AL-140; GLP-1 ELISA AL-172; GLP-2 ELISA AL-174; Follistatin ELISA AL-117; Oxyntomodulin ELISA AL-139; Glucagon ELISA AL-157
Calvert ME, Kalra B, Patel A, Kumar A, Shaw ND. Serum and urine profiles of TGF-β superfamily members in reproductive aged women. Clin Chim Acta. 2021 Dec 5;524:96-100. doi: 10.1016/j.cca.2021.12.002. Online ahead of print
All Products Cited: Inhibin A (pico) ELISA AL-184; Inhibin B ELISA AL-107; Inhibin (Total) ELISA AL-134; AMH (PCOCheck) ELISA AL-196; Activin A ELISA AL-110; Activin B ELISA AL-150; Activin AB ELISA AL-153; Follistatin ELISA AL-117; GDF-9/BMP-15 Complex ELISA AL-181; GDF-15 (Total) ELISA AL-1014; GDF-15 (H2O2D non-detectable) ELISA AL-1018
Perakakis N, Chen J, Ghaly W, Vamvini MT, Mantzoros CS. Effects of oral and intravenous lipid administration on hormones affecting energy balance. Poster session presented at the Obesity Week Meeting; 2017 Oct 29-Nov 2; Washington DC.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin Like 3 ELISA AL-152; Oxyntomodulin ELISA AL-139
Perakakis N, Mougios V, Fatouros I, Siopi A, Draganidis D, Peradze N, Ghaly W, Mantzoros CS. Physiology of Activins/Follistatins: Associations With Metabolic and Anthropometric Variables and Response to Exercise. J Clin Endocrinol Metab. 2018 Oct 1;103(10):3890-3899.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117; Follistatin-like 3 ELISA AL-152
Panagiotou G, Papakonstantinou E, Vagionas A, Polyzos SA, Mantzoros CS. Serum Levels of Activins, Follistatins, and Growth Factors in Neoplasms of the Breast: A Case-Control Study. J Clin Endocrinol Metab. 2019 Feb 1;104(2):349-358.
All Products Cited: Activin A ELISA AL-110; Activin B ELISA AL-150; Follistatin ELISA AL-117; Follistatin Like 3 ELISA AL-152; IGF-I ELISA AL-121; Total IGFBP-4 ELISA AL-126; Intact IGFBP-4 ELISA AL-128